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Duration 14 hours
Course Outline
Introduction to Fiji and ImageJ
- Overview of Fiji and its role in scientific research
- Understanding the architecture of ImageJ and its plugin ecosystem
- Installation and configuration of Fiji for optimal performance
Basic Image Processing Techniques
- Image acquisition and supported import formats
- Adjusting contrast and brightness, and applying standard filters
- Methods for image stacking and projection
Advanced Image Analysis
- Segmentation techniques, including thresholding and edge detection
- Object counting and particle analysis within histological samples
- Measuring areas, perimeters, and other morphological features
Working with Biological Samples
- Processing images of histological tissues, blood cells, and algae
- Color deconvolution and analysis of stained tissue sections
- Time-lapse analysis and tracking of cell movement
Automation and Customization
- Developing and executing macros to automate repetitive tasks
- Utilizing Fiji plugins for specialized analytical procedures
- Introduction to scripting using the ImageJ Macro Language (IJM)
Data Management and Reporting
- Exporting results and generating comprehensive reports
- Annotating images and preparing data for publication
- Best practices for ensuring reproducible image analysis
Requirements
- Fundamental knowledge of biological imaging concepts
- Experience with microscopy and histological techniques
- Basic computer literacy
Target Audience
- Researchers in biotechnology and environmental toxicology
- Laboratory technicians engaged in histological and cellular imaging
- Scientists with an interest in quantitative image analysis
Custom Corporate Training
Training solutions designed exclusively for businesses.
- Customized Content: We adapt the syllabus and practical exercises to the real goals and needs of your project.
- Flexible Schedule: Dates and times adapted to your team's agenda.
- Format: Online (live), In-company (at your offices), or Hybrid.
Price per private group, online live training, starting from 2600 € + VAT*
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